Nevertheless, there was no commercial solution until AES introduced a breakthrough innovation recently.
AES developed a proprietary column diameter transformation cartridge (patent pending), which has a large inner diameter (ID) separation capillary, and a small ID transfer capillary. Once the proteins are separated, they are forced into the transfer capillary with minimal peak remixing.
The effectiveness of this proprietary cartridge is illustrated with iCIEF separation, individual peak collection, and iCIEF of the collections. The individual peaks were collected at 2 minute intervals, and the fraction was mixed with a carrier ampholytes solution. The iCIEF results of the individual fractions show the efficiency of a 2 minute collection. Mingling of neighboring peaks can be minimized with shorter collection intervals.