Analytical iCIEF

Imaged capillary isoelectric focusing (iCIEF) is a high-resolution technique for separating protein mixtures into groups based on their isoelectric point (pI). Compared to conventional CIEF systems, iCIEF provides faster method development, higher detection sensitivity, better reliability, and higher analytical throughput.

The CEInfinite Analytical iCIEF instrument accepts methods from other iCIEF instruments with no additional method development required. However, separation resolution is improved and using CEInfinite whole column imaging detection (WCID) cartridges with the same method can increase detection sensitivity.

In a study from the University of Waterloo published in the Journal of Chromatography B, CEInfinite iCIEF was used to characterize salivary α-amylase collected from various glands and prepared using methods. The pIs observed using the CEInfinite agreed with those reported in the literature with a high level of precision. This study shows the accuracy of CEInfinite iCIEF to resolve and quantitate enzyme samples.

iCIEF Instrument

All CEInfinite iCIEF instruments have a high sensitivity CMOS imaging sensor and powerful deep UV LED light source; this increases the scan rate over 100-fold compared to any other iCIEF systems and allows for the study of dynamic processes.

The autosampler for the CEInfinite Analytical instrument comes in an 84+3 or 96-well plate configuration, the latter of which is capable of on-board mixing. The CEInfinite Analytical was featured in an issue of Chromatography Today, where the reproducibility of on-board mixing was shown to be greater than manual mixing of samples.

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